... peptides inthedairyindustry 12.8 Treatment ofeffluentsandtechnicalfluidsinthedairyindustry 12.9 Conclusions and future trends inthedairyindustry 12.10 The egg ... inthe hydrostatic pressure However, inthe presence of dense gas, the melting point ofthe solid decreases as the pressure increases owing to the increasing solubility of gas inthe solid The ... branches; one going from the critical point ofthe component with the higher critical temperature, initially inthe direction ofthe critical point ofthe other component and then rising sharply...
... apoptosis-inhibiting function, andthe further addition ofthe residue 270–401-containing PEST2 and RING finger domain resulted in rapid proteolysis Therefore, the apoptosis-inhibiting potential of xXIAP ... (compare Figs 5A and 6C) Thus, in both xEIAP ⁄ XLX and xXIAP, the C-terminal region containing the RING finger domain is required for the initial phase of proteolysis within h and also for the final degradation ... (PEST2) and RING finger domain of xEIAP ⁄ XLX, andthe first PEST sequence (PEST1) is further deleted in ED2 (Fig 6A) Similarly, the XD1 mutant lacks the RING finger domain and C-terminal half of the...
... other common anthocyanin containing fruits and vegetables, the concentration of anthocyanins in purple-fleshed ISPs are inthe same range as some ofthe highest anthocyanin producing crops like blueberries, ... In this case, the main effects of solvent and extraction temperature, the interaction between solvent and temperature and solvent and solid loading, as well as the full interaction of all three ... Change in AIS was used to represent the change in starch content as a percent of dry matter and was examined in this study to determine the amount of starch converted during hydrolysis In this...
... viral minor capsid proteins The results may facilitate a better understanding ofthe molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection inthe future ... Identification of VP51 and VP76 by Western blotting The truncated recombinant proteins, VP51p and VP76p, were expressed in Escherichia coli and purified by using NiNTA affinity chromatography, and then ... any remaining bound proteins The low-pH treated sample was suspended in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV...
... is the synthesis ofthe oogenic proteins, Vtg and Zrproteins E2 is the major estrogen in female fish E2 stimulates the production of Vtg and Zr-proteins inthe liver The genes encoding these fish ... protein synthesis Eggshell zona radiata proteins andthe egg yolk protein precursor, vitellogenin are synthesized and secreted by the hepatocyte They are transported in blood to the ovary and incorporated ... for the silencing of at least nine ofthe ten pseudogenes (ibid.) It has become increasingly clear that the proteins ofthe zona pellucida are conserved among eutherian mammals and that the proteins...
... domain The fusion plasmids are then cloned into the yeast cell and expressed on the cell membrane If the prey protein interacts with the bait protein, this would bind the NubG and Cub domains ... shown in Fig indicated that the cDNA library constructed in this study was suitable for the screening of cellular proteins interacting with HBsAg Figure Determination of Size of Inserts in cDNA ... coxackie B virus and involved inthe transcytosis ofthe virus [17] Further functional analysis of these HBsAginteracting cellular membrane proteins should shed new insights on their role in HBV morphogenesis...
... demonstrated the same mobility in both stains, suggesting that these bands were complexes of protein and saccharide These included the band corresponding to a molecular weight of 103,000 inthe steaming-extracted ... (1), the band corresponding to 38,000 inthe NaOH-extracted polymer (2), the band corresponding to 24,700 inthe SDS-extracted polymer (3), andthe bands corresponding to 10,700, 29,200, and 39,000 ... was determined according to the method of Lowry et al (1951) using a bovine serum albumin standard Nucleic acid and protein contents inthe gel filtration eluate were determined by measuring absorbances...
... along the lines to create the boat The order and directions for folding are documented inthe functional specification After completing the above steps, you will discuss the activity with the class ... Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct an origami boat using the sheet distributed by the instructor Fold the paper along the ... steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...
... protein mutants and RNA containing cytosine at position )5 (C) The characteristic intramolecular hydrogen bond between the amino group of C5 andthe O1P atom of U6 observed inthe structure ofthe ... test Starting with each complex inthe training set containing one or more RRM or KH domains, we extracted the protein coordinates andthe four nucleotides bound at the center ofthe domain (for ... capable of predicting the specificity of RNA-binding proteins across entire families (such as the RRM superfamily), or of redesigning the specificity of these proteins, would be of equal importance in...
... diagram ofthe constructs microinjected into the medaka eggs and transfected into HeLa cells The olvas, nanos and tudor coding sequences were joined to the C-terminal region ofthe GFP or RFP coding ... measure the mobility ofthe components inthe PGCs The medaka embryo was peeled off the chorion, andthe segment containing the part of PGCs was excised for observation by microscopy and FCS ... photobleaching (FRAP) are often used to assess the dynamics and kinetic properties of proteins in living cells [1219] FCS detects the uctuations of uorescent intensity derived from the movement of a single...
... accurate definition ofthe C-terminal domain boundary Experimental Study ofthe interactions ofthe Anp32a LRR module determination ofthe dynamic features ofthe domain in solution, together with a ... with the aim of mapping the surface of interaction between the two proteins This method, which relies on the effect that binding of a molecule has on the electron distribution of another, causing ... perturbation of its NMR spectrum, is routinely used to detect interactions and map them on the structures ofthe individual components Fig Probing the interaction between the Anp32a LRR domain and the...
... composed ofthe sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure andthe function of these proteins Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... based on the conserved amino acid sequences ofthe catalytic domain of members ofthe GH18 family resulted inthe amplification of an expected 147 bp sequence Cloning and sequencing of this fragment ... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved inthe control of growth and remodelling processes in a...
... determined using serial dilutions of samples (Fig 2) Linearity consisting of continuous signal increase andof reproducible glycoprotein patterns was determined inthe range between and 10 lL of ... analysis of brain tissues obtained from cattle After immunoblotting, PrPC signals were detected using the antibodies indicated (B) The protein banding pattern ofthe three PrPC protein bands, the ... The signal intensity of each ofthe three protein bands was quantified by densitometry The mean values ofthe calculated signal intensities were analyzed for each ofthe N- or C-terminal binding...
... downstream ofthe initiator ATG in exon (Ex1) ofthe 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt andthe release of an N-terminal fragment bearing the ... analyzed The arrow indicates the position ofthe more intensively oxidized polypeptide inthe assay The bracket underlines the domain (molecular mass range 10–40 kDa) ofthe oxyblots that contains the ... 1B,Cc,D) In both cases (72Q and 103Q), a broad distribution ofthe size ofthe inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution ofthe size of...
... N-terminal domains (N1, N2) ofthe minor coat protein (g3p) are responsible for binding and infection in E coli Thus, incorporation of a library of target proteins between the N-terminal domain and ... protein inthe absence of any cofactors In this work, the authors used the method similar to that of Finucane et al [11] except that the protein A B-domain instead of His-tag was used to select the ... library of target proteins with a tag can be fused to the g3p protein on the surface ofthe phage The tag can be a His-tag [11] or antibodybinding proteins such as the protein AB-domain [18]...
... immunoblotting data, it was of interest to re-examine the fate of calsequestrin by studying the entire complement of key Ca2+-binding elements in dystrophindeficient skeletal muscle fibres Because the ... tracking dye] to achieve a final protein concentration of 50 lg of protein per IEF strip for silver staining, hot Coomassie staining, ÔStains-AllÕ labelling or immunoblotting The following running ... combination ofthe pI value and molecular mass of individual 2D spots can be useful inthe initial identification of proteins However, owing to the abnormal electrophoretic mobility of certain...
... Portland, Oregon ACL Minqing Hu and Bing Liu 2004 Mining and summarizing customer reviews In Proceedings ofthe tenth ACM SIGKDD international conference on Knowledge discovery and data mining, ... study the effect of different parameter settings There are several parameters inthe framework: the number of generated seeds r, the number of new candidates k2 andthe number of selections k in ... the proposed two-stage domain adaptation framework: 1) generating some sentiment and topic seeds inthe target domain; and 2) expanding the seeds inthe target domain to construct sentiment and...
... list of phrases split into subsets (preceding vs following their scope) to identify cues using string matching The cue scopes extend from the cue to the beginning or end ofthe sentence, depending ... Statistics ofthe BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% ofthe ... by matching the path of cue leaf nodes to the root ofthe rule subtree pattern If an identical path exists inthe sentence, the root ofthe candidate subtree is thus also identified The candidate...
... described in (Asahara and Matsumoto, 2003) and features of surrounding phrases described in (Nakano and Hirai, 2004) We will in1 28 vestigate incorporating SF and these features inthe near future ... vector Vm of a morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair ofthe surface string andthe part -of- speech of mi The similar ... morphemes, and (Asahara and Matsumoto, 2003; Nakano and Hirai, 2004) formalized the task of extracting NEs as a chunking problem of a sequence of characters instead of a sequence of morphemes In this...
... structure relies mainly on the evidence outlined above In view ofthe close similarity ofthe ROA spectra ofthe casein, synuclein and tau proteins shown in this paper, we have reproduced in Fig from ... that the former is likely to be inthe Cterminal half andthe latter inthe N-terminal half ofthe protein [29,48±51] Sequence-based structure prediction methods suggest that the caseins are ofthe ... form of b strand [18] The positive bands at » 1675 cm)1 inthe amide I region ofthe ROA spectra of b- and j-casein, which originate mainly inthe peptide C O stretch, are characteristic of disordered...